PlanTherm PT 200

PlanTherm PT 200 enables fast and easy estimation of heat tolerance of various plants, plant suspension cells, algae, and cyanobacteria. PlanTherm PT200 simultaneously monitors conductivity and chlorophyll a fluorescence of the analyzed sample during its gradual heating from 25 to 75°C in less than 1 hour. The heating process is controlled, recorded and evaluated by PC with intuitive ProfileCon software.

Sample chamber for small up to middle site leaves, leaf segments, algae or cyanobacteria suspensions.

Precisely controlled gradual (linear) heating of plant segments with the slope up to 3 °C/min.

Accurate temperature measurements (±0.3 °C) within the range of 20 – 75 °C.

Conductivity measurement within 1 – 1,000 µS/cm

Measurement of the minimal chlorophyll a fluorescence intensity (F t level)

Compact device including measuring unit, magnetic stirrer, thermoregulator in one case.

Intuitive graphic interface ProfileCon and built-in scripting (R environment) language allows custom analysis of measured responses in control PC.

PlanTherm PT 200 PlanTherm PT 200 enables fast and easy estimation of heat tolerance of various plants, plant suspension cells, algae, and cyanobacteria (Bednarikova et al., 2020, Chen et al., 2023, Ilek et al., 2018, Zhang, et al., 2019). As the heat stress leads to disintegration of cell membranes and so to increased ion leakage from cytosol to solvent, PlanTherm PT200 simultaneously monitors conductivity and chlorophyll a fluorescence of the analyzed sample during its gradual heating from 25 to 75°C in less than 1 hour (Ilek et al., 2018). Whereas the change in conductivity is related to the cell membrane thermostability, the chlorophyll a fluorescence intensity (so called minimal fluorescence level – Ft) is connected to primary photosynthetic processes (thermostability of thylakoid membranes and function of the photosystem II). The measured conductivity-temperature curve enables to determine the exact threshold temperature when leakage of ions from cells occurs. The threshold temperature is taken as a measure of the heat stability of studied plant life-form. PlanTherm device is designed for analysis of various cell suspensions and plant material ranging from small up to middle size leaves or leaf segments. The heating process is controlled, recorded and evaluated by PC with intuitive ProfileCon software.

PlanTherm PT 200 is provided with a laptop with preinstalled Windows 11 operation system. The new intuitive user-interface ProfileCon v3 allows to setup experiment, analyze data and export measured curves to textual output file. Plots of currently running experiment, measured raw data and analyzed results can be displayed on the main screen.

PlanTherm PT 200 can be used in basic photosynthetic research, in agriculture, and horticulture for: fast estimations of both the constitutive and inducible heat tolerance of plants, algae and cyanobacteria quick measurements of thermal stability of cell membranes studies of stress tolerance and acclimation responses - detection of organism’s resistance or susceptibility to given stress factors, detection of biotic and abiotic stress screenings of transgenic lines

fast estimations of both the constitutive and inducible heat tolerance of plants, algae and cyanobacteria

quick measurements of thermal stability of cell membranes

studies of stress tolerance and acclimation responses - detection of organism’s resistance or susceptibility to given stress factors, detection of biotic and abiotic stress

screenings of transgenic lines

Preinstalled Windows 11 operation system

ProfileCon v3 graphical user-interface as a control and visualization software

R software environment for running the custom analysis on measured signals

Optional Sample Holder for PlanTherm PT 200 Includes sample holder and sample holder cube, Allen wrench

Spare Cuvette for PlanTherm PT 200 Cuvette 402.000-OG 10mm

Combined Temperature/conductivity Probe for PlanTherm PT 200 PlanTherm sensor PT1000/MV1

Magnetic Stirrer Bar for PlanTherm PT 200 Magnetic stirrer bar PTFE 3x8 mm

Pricing PlanTherm Version Characteristics Price PlanTherm PT 200 PT 200, a compact system for fast and easy estimation of heat stability of algae and plants + PC (Win11, Memory RAM 12 GB, Storage 256 GB SSD + 1 TB HDD, Processor > i3) 12,790 €

Spare components Sample Holder Includes sample holder, sample holder cube and Allen wrench 99 € Spare Cuvette Cuvette 402.000-OG 10mm 165 € Combined Temperature-conductivity Probe PlanTherm sensor 490 € Magnetic Stirrer Bar for PlanTherm PT 200 Magnetic stirrer bar PTFE 3 × 8 mm 10 €