Closed FluorCam

Closed FluorCam FC 800-C is a popular, best-selling system for imaging chlorophyll fluorescence kinetics. It features an integrated dark chamber for accurate and controlled fluorescence measurements. The system is available in multiple configurations, including a multicolor module for capturing diverse fluorescent signals, and a GFP-optimized version for steady-state fluorescence studies. The standard configuration includes a 1.4 MPx CCD camera and four fixed LED panels, providing uniform illumination across sample areas up to 90 × 90 mm—ideal for small plants, detached leaves, algal suspensions, and similar specimens.

Four high-intensity LED panels available in several wavelengths

High-resolution imaging with two camera options: either 1.4 MPx TOMI-2: 6.45 µm × 6.45 µm pixel size (Standard high-resolution camera) or 1.3 MPx TOMI-3: 6.60 µm × 6.60 µm pixel size (Fast camera for OJIP measurements)

Built-in dark chamber for light-controlled measurements

Adjustable shelf system to accommodate various plant sizes

Choice of imaging masks for 384-well plates, 96-well plates, Petri dishes, and more

Delivered with a laptop and fully integrated control and analysis software

Optional top-mounted LED panel for expanded excitation capabilities

Closed FluorCam and GFPCam FC 800-C

The FC 800-C is a robust, user-friendly, and world-wide used system for combined multispectral and kinetic fluorescence imaging. Designed for high-performance and versatility, it features a CCD camera, four fixed LED panels (with an optional fifth top-mounted panel), and the option to add a motorized filter wheel with up to seven emission filters.

The LED panels provide uniform illumination across a 90 × 90 mm area—ideal for imaging small plants (e.g., Arabidopsis thaliana ), detached leaves, mosses, lichens, plated algal colonies, or algal suspensions. Its compact design includes an integrated dark chamber for easy and effective dark adaptation of samples.

The FC 800-C captures fluorescence images at any time during the experiment, presenting data as false-color maps for intuitive visualization. Full kinetic analysis is supported, including fluorescence transients induced by actinic light or saturating flashes, with customizable timing and intensity settings via user-defined protocols.

The system comes complete with a high-performance laptop and a comprehensive software suite FluorCam10 for system control, image acquisition, and data analysis. For advanced users, the software also optionally enables automated triggering of protocols via the Advanced Function software extension. Light Panels The standard FluorCam FC 800-C system includes four high-intensity LED panels, each measuring 130 × 130 mm. One pair provides Measuring Light and Actinic Light 1 (standard & GFPCam: red-orange, 619 - 626 nm), while the other pair delivers Actinic Light 2 and Saturating Pulses (standard: cool white 6 500 K CCT; GFPCam: Royal blue, 444 - 464 nm). Alternative wavelengths are available in the customized FC 800-C/1010-C as replacements for the standard Actinic Light 2 and Saturating Pulse panels in custom configurations. These include: Blue (460 - 470 nm) Cyan (495 - 510 nm) Green (525 - 530 nm) Red (619 - 626 nm) Deep red (635 - 666 nm) Amber (585 - 595 nm) An optional top-mounted LED panel can be added to expand the excitation spectrum for multicolor fluorescence or reflectance measurements. This panel is mounted around the camera and is available in the following wavelengths: Far-red (720 - 740 nm) Deep-red (635 - 666 nm) Ultraviolet A (standard: 380 – 390 nm, optional: 360 – 370 nm, or 400 – 410 nm) Ultraviolet B (303 – 317 nm) Other wavelengths available upon request. Light Intensity Specifications Actinic light intensity: - standard version: 300–2 000 µmol·m⁻²·s⁻¹ (depending on wavelength) - light-upgraded version: up to 3 000 µmol·m⁻²·s⁻¹ (please inquire) Saturating pulse intensity: - standard version: up to 4 000 µmol·m⁻²·s⁻¹ - light-upgraded version: up to 6 000 µmol·m⁻²·s⁻¹ (please inquire) Single Turnover Flash (STF): - 120 000 µmol·m⁻²·s⁻¹ in a 100 µs pulse (available in QA re-oxidation version) Camera options Standard version: high-resolution CCD camera TOMI-2: 6.45 µm × 6.45 µm pixel size, frame period 50 ms OJIP version: fast CMOS camera TOMI-3: 6.60 µm × 6.60 µm pixel size, frame period 20 - 2000 µs (please inquire) Predefined Protocols

Ultraviolet A (standard: 380 – 390 nm, optional: 360 – 370 nm, or 400 – 410 nm)

Ultraviolet B (303 – 317 nm)

Other wavelengths available upon request.

Saturating pulse intensity:

Single Turnover Flash (STF):

Standard version: high-resolution CCD camera TOMI-2: 6.45 µm × 6.45 µm pixel size, frame period 50 ms

OJIP version: fast CMOS camera TOMI-3: 6.60 µm × 6.60 µm pixel size, frame period 20 - 2000 µs (please inquire)

For use with the standard high-resolution TOMI-2 camera FV/FM - Maximum quantum yield of photosystem II Kautsky Induction - Time-resolved fluorescence response following dark adaptation Quenching Analysis — Differentiation of photochemical and non-photochemical quenching Light Curve —Measurement of photosynthetic responses across light intensities Steady state fluorescence , e.g., ChlF, optionally GFP Multicolor Fluorescence Imaging — Detection of UV-induced blue (F440), green (F520), and chlorophyll fluorescence (F680, F740), requires optional accessories: an additional UV A LED panel, and filter wheel with 4 filters QA-Reoxidation Kinetics — Time-resolved analysis of electron transport chain recovery, requires optional electronic module Fast Fluorescence Induction (OJIP) — Fast chlorophyll a fluorescence induction transient recorded by a method of multiple-flashes, requires optional electronic module PAR Absorptivity Measurement — Estimation of photosynthetically active radiation absorption based on NDVI, requires optional accessories: an additional NDVI (deep red/far-red) LED panel, and a filter wheel with a glass filter For use with the fast TOMI-3 camera:

FV/FM - Maximum quantum yield of photosystem II

Kautsky Induction - Time-resolved fluorescence response following dark adaptation

Quenching Analysis — Differentiation of photochemical and non-photochemical quenching